QP Lab.

Uyeda Lab.
Molecular Biophysics

Waseda Univ. School of Advanced Science & Engineering, Department of Physics


Miyata M, Robinson RC, Uyeda TQP, Fukumori Y, Fukushima S, Haruta S, Homma M, Inaba K, ItoM, Kaito C, Kato K, Kenri T, Kinosita Y, Kojima S, Minamino T, Mori H, Nakamura S, Nakane D, Nakayama K, Nishiyama M, Shibata S, Shimabukuro K, Tamakoshi M, Taoka A, Tashiro Y, Tulum I, Wada H, Wakabayashi K (2020)
Tree of Motility – A Proposed History of Motility Systems in the Tree of Life.
Genes Cells, 25:6-21 doi: 10.1111/gtc.12737


Fujimoto K, Tanaka M, Rana AYKMM, Jahan MGS, Itoh G, Tsujioka M, Uyeda TQP, Miyagishima SY, Yumura S. (2019)
Dynamin-like protein B of Dictyostelium discoideum contributes to cytokinesis cooperatively with other dynamins.
Cells 26:8. doi: 10.3390/cells8080781.

Tsujioka M, Uyeda TQP, Iwadate Y, Patel H, Shibata K, Yumoto T, Yonemura S. (2019)
Actin-binding domains mediate the distinct distributions of two Dictyostelium talins through different affinities to specific subsets of actin filaments during directed cell migration.
PLoS One, 14: e0214736. doi: 10.1371/journal.pone.0214736

Umeki N, Shibata K, Noguchi TQP, Hirose K, Sako Y, Uyeda TQP. (2019)
K336I mutant actin alters the structure of neighboring protomers in filaments and reduces affinity for actin-binding proteins.
Sci. Rep., 9:5353. doi: 10.1038/s41598-019-41795-w.


Kijima, S.T., Staiger, C.J., Katoh, K., Nagasaki, A., Ito, K., and Uyeda, T.Q.P. (2018)
Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells.
Sci Rep., 8:4381. doi: 10.1038/s41598-018-22707-w.

Kuragano, M., Uyeda, T.Q.P., Kamijo, K., Murakami, Y., and Takahashi, M. (2018)
Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.
Mol. Biol. Cell., 29:911-922. doi: 10.1091/mbc.E17-04-0215.

Rula, S., Suwa, T., Kijima, S.T., Haraguchi, T., Wakatsuki, S., Sato, N., Duan, Z., Tominaga, M., Uyeda, T.Q.P., and Ito, K. (2018)
Measurement of enzymatic and motile activities of Arabidopsis myosins by using Arabidopsis actins.
Biochem. Biophys. Res. Commun., 495:2145-2151. doi: 10.1016/j.bbrc.2017.12.071.


Shen, Y., Cheng, Y., Uyeda, T. Q. P., Plaza, G. R. (2017)
Cell Mechanosensors and the possibilities of using magnetic nanoparticles to study them and to modify cell fate.
Annal Biomed. Eng., doi: 10.1007/s10439-017-1884-7. [Epub ahead of print]

Iwase, K., Tanaka, M., Hirose, K., Uyeda, T.Q.P. and Honda, H. (2017)
Acceleration of the sliding movement of actin filaments with the use of a non-motile mutant myosin in in vitro motility assays driven by skeletal muscle heavy meromyosin.
PLoS One, 12:e0181171. doi: 10.1371/journal.pone.0181171.

Hirakawa, R., Nishikawa, Y., Uyeda, T.Q.P., Tokuraku, K. (2017)
Unidirectional growth of heavy meromyosin (HMM) clusters along actin filaments revealed by real-time fluorescence microscopy.
Cytoskeleton, doi: 10.1002/cm.21408. [Epub ahead of print]

Nagasaki, A., Kijima, S.T., Yumoto, T., Imaizumi, M., Yamagishi, A., Kim, H., Nakamura, C., Uyeda, T.Q.P. (2017)
The position of the GFP tag on actin affects the filament formation in mammalian cells.
Cell Struct. Funct., 42:131-140. doi: 10.1247/csf.17016

Shen, Y., Wu, C, Uyeda, T. Q. P., Plaza, G. R., Liu, B., Han, Y., Lesniak, M. S. and Cheng, Y. (2017)
Elongated Nanoparticle Aggregates in Cancer Cells for Mechanical Destruction with Low Frequency Rotating Magnetic Field.
Theranostics, 7:1735-1748


Shibata, K., Nagasaki, A., Adachi, H., and Uyeda, T.Q.P. (2016)
Actin binding domain of filamin distinguishes posterior from anterior actin filaments in migrating Dictyostelium cells.
Biophys Physicobiol., 13:321-331

Ngo, KX., Umeki, N., Kijima, ST., Kodera, N., Ueno, H., Furutani-Umezu, N., Nakajima, J., Noguchi, T.Q.P., Nagasaki, A., Tokuraku, K. and Uyeda, T.Q.P. (2016)
Allosteric regulation by cooperative conformational changes of actin filaments drives mutually exclusive binding with cofilin and myosin.
Scientific Reports, 6:35449

Huang,S., Umemoto,R., Tamura,Y., Kofuku,Y., Uyeda, T.Q.P., Nishida, N., and Shimada,I. (2016)
Utilization of paramagnetic relaxation enhancements for structural analysis of actin-binding proteins in complex with actin.
Scientific Reports, 6:33690

Umeki, N., Hirose, K., and Uyeda, T.Q.P. (2016)
Cofilin-induced cooperative conformational changes of actin subunits revealed using cofilin-actin fusion protein.
Scientific Reports, 6:20406

Kijima, S.T., Hirose, K., Kong, S.G., Wada, M., and Uyeda, T.Q.P. (2016)
Distinct Biochemical Properties of Arabidopsis thaliana Actin Isoforms.
Plant Cell Physiol, 57(1):46–56


Plaza, G.R., Uyeda, T.Q.P., Mirzaei, Z., and Simmons, C.A.
Study of the influence of actin-binding proteins using linear analyses of cell deformability.
Soft Matter., 11:5435-5446

Noguchi, T.Q.P., Morimatsu, M., Iwane, A.H., Yanagida, T. and Uyeda, T.Q.P.
The Role of Structural Dynamics of Actin in Class-Specific Myosin Motility.
PLoSOne, in press.

Ohnuki-Nagasaki, R., Nagasaki, A., Hakamada, K., Uyeda, T.Q.P., Miyake, M., Miyake, J., and Fujita, S. (2015)
Identification of kinases and regulatory protiens required for cell migration using a transfected cell-microarray system.
BMC Genomics, 16:9.

Ngo, K.X., Kodera, N., Katayama, E., Ando, T., and Uyeda, T.Q.P. (2015).
Cofilin-induced unidirectional cooperative conformational changes in actin filaments revealed by high-speed atomic force microscopy.
eLife, 2015;4:e04806.   free access


上田太郎 (2014)
生物物理. 52:100-104.   free access


Gomibuchi, Y., Uyeda, T. Q. P., Wakabayashi, T. (2013)
Bulkiness or aromatic nature of tyrosine-143 of actin is important for the weak binding between F-actin and myosin-ADP-phosphate.
Biochem. Biophys. Res. Commun. 441:844-848.

Plaza, G. R., and T. Q. P. Uyeda (2013).
Contraction speed of the actomyosin cytoskeleton in the absence of cell membrane.
Soft Matter, 9:4359-4554.

Shiozaki, N., Nakano, K., Kushida, Y., Noguchi, T.Q.P., Uyeda, T.Q.P., Wloga, D., Dave, D., Vasudevan, K. K., Gaertig, J., and Numata, O. (2013)
ADF/cofilin is not essential but is crucially important for actin activities during phagocytosis in Tetrahymena thermophila.
Eukaryotic Cell 12:1080-1086.


Umeki, N., J. Nakajima, J., T. Q. P. Noguchi, K. Tokuraku, A. Nagasaki, K. Ito, K. Hirose and T. Q. P. Uyeda (2012).
Rapid Nucleotide Exchange Renders Asp11 Mutant Actins Resistant to Depolymerizing Activity of Cofilin, Leading to Dominant Toxicity in vivo.
J. Biol. Chem., 288:1739-1749.   free access

Noguchi, T. Q. P., T. Komori, N. Umeki, N. Demizu, K. Ito, A. Hikikoshi-Iwane, K. Tokuraku, T. Yanagida and T. Q. P. Uyeda (2012).
G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.
J. Biol. Chem., 287:24339-24345.   PubMed


Uyeda, T. Q. P., Y. Iwadate, N. Umeki, A. Nagasaki, and S. Yumura (2011).
Stretching actin filaments within cells enhances their affinity for the myosin II motor domain.
PLoS One, 6:e26200.   free access

Umeki N, and T. Q. P. Uyeda (2011).
Single restriction enzyme-assisted megaprimer polymerase chain reaction to fuse two DNA sequences on separate cloning vectors.
Anal. Biochem., 414:309-311.   PubMed


Murakami, K., T. Yasunaga, T. Q. P. Noguchi, Y. Gomibuchi, K. X. Ngo, T. Q. P. Uyeda, and T. Wakabayashi (2010).
Structural Basis for Actin Assembly, Activation of ATP Hydrolysis, and Delayed Phosphate Release.
Cell, 143:275-287.   PubMed

Iwai, S., and T. Q. P. Uyeda (2010).
Myosin-actin Interaction in Dictyostelium Cells Revealed by GFP-based Strain Sensor and Validated Linear Spectral Unmixing.
Cytometry A,77:743-750.   free access

Noguchi, T. Q. P., R. Toya, H. Ueno, K. Tokuraku, and T. Q. P. Uyeda (2010).
Screening of novel dominant negative mutant actins using glycine targeted scanning identified G146V actin that cooperatively inhibits cofilin binding.
Biochem. Biophys. Res. Commun., 396:1006-1011.   PubMed

Takatsuki, H., K. M. Rice, S. Asano, B. S. Day, M. Hino, K. Oiwa, R. Ishikawa, Y. Hiratsuka, T. Q. P. Uyeda, K. Kohama, and E. Blough (2010).
Utilization of myosin and actin bundles for the transport of molecular cargo.
Small, 15:452-457.   PubMed

Hachikubo, Y., S. Iwai, and T. Q. P. Uyeda (2010).
Photoregulated assembly/disassembly of DNA-templated protein arrays using modified oligonucloetide carrying azobenzene side chains.
Biotech. Bioeng., 106:1-8.   PubMed

Suetsugu, N., N. Yamada, T. Kagawa, H. Yonekura, T. Q. P. Uyeda, A. Kadota, and M. Wada M (2010).
Two kinesin-like proteins mediate actin-based chloroplast movement in Arabidopsis thaliana.
Proc. Natl. Acad. Sci. USA., 107:8860-8965.   free access

Onuki-Nagasaki, R., A. Nagasaki, K. Hakamada, T. Q. P. Uyeda, S. Fujita, M. Miyake, and J. Miyake (2010).
Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
Methods Mol. Biol. 629:193-203.   PubMed


Noguchi, T. Q. P., Y. Gomibuchi, K. Murakami, H. Ueno, K. Hirose, T. Wakabayashi, and T. Q. P. Uyeda (2009).
Dominant negative mutant actins identified in flightless Drosophila can be classified into three classes, including perturbation of the tropomyosin-troponin system.
J. Biol. Chem., 285:4337-4347.   free access

Kanada, M., A. Nagasaki, and T. Q. P. Uyeda (2009).
Stabilization of Anaphase Midzone Microtubules is Regulated by Rho during Cytokinesis in Human Fibrosarcoma Cells.
Exp. Cell Res. 315:2705-2714.   PubMed

Nagasaki, A., M. Kanada, and T. Q. P. Uyeda (2009).
Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum.
Cell Res. 19:236-246.   free access

Tokuraku, K., R. Kurogi, R. Toya, and T. Q. P. Uyeda (2009).
Novel mode of cooperative binding between myosin and Mg2+-actin filaments in the presence of low concentrations of ATP.
J. Mol. Biol. 386:149-162.   PubMed

Kamei, T., H. Akiyama, H. Morii, N. Tamaoki, and T. Q. P. Uyeda. (2009)
Visible-Light Photocontrol of (E)/(Z) Isomerization of the 4-(Dimethylamino)azobenzene Pseudo-Nucleotide Unit Incorporated into an Oligonucleotide and DNA Hybridization in Aqueous Media.
Nucleos. Nucleot. Nucl. 28:12-28.   PubMed

長崎晃、長崎玲子、藤田聡史、上田太郎 (2009)
生化学 81:381-386.


Nagasaki, A, M. Kanada, and T. Q. P. Uyeda (2007).
A novel shRNA vector that enables rapid selection and identification of knockdown cells.
Plasmid 58:190-194.   PubMed

Noguchi T. Q. P., N. Kanzaki, H. Ueno, K. Hirose, and T. Q. P. Uyeda (2007).
A novel system for expressing toxic actin mutants in Dictyostelium and purification and characterization of a dominant lethal yeast actin mutant.
J. Biol. Chem. 282:27721-27727.   free access

Kamei, T., M. Kudo, H. Akiyama, M. Wada, J. Nagasawa, M. Funahashi, N. Tamaoki, and T. Q. P. Uyeda (2007).
Photoresponsivity to visible light of 4-dimethylaminoazobenzene incorporated into single-stranded DNA: demonstration of a large spectral change accompanying isomerization in DMSO and detection of rapid cis-to-trans isomerization in aqueous solution.
Eur. J. Org. Chem. 2007:1846-1853.


Hiratsuka, Y., M. Miyata, T. Tada, and T. Q. P. Uyeda (2006).
A microrotary motor powered by bacteria.
Proc. Natl. Acad. Sci. USA. 103:13618-13623.   free access

Yonekura, H., A. Nomura, H. Ozawa, Y. Tatsu, N. Yumoto, and T. Q. P. Uyeda (2006).
Mechanism of tail-mediated inhibition of kinesin activities studied using synthetic peptides.
Biochem. Biophys. Res. Commun. 343:420-427.   PubMed

Kanzaki, N., T. Q. P. Uyeda, and K. Onuma (2006).
Intermolecular interaction of actin revealed by a dynamic light scattering technique.
J. Phys. Chem. B 110:2881-2887.   PubMed

Konishi K., T. Q. P. Uyeda, and T. Kubo (2006).
Genetic engineering of a Ca2+ dependent chemical switch into the linear biomotor kinesin.
FEBS Lett. 580:3589-3594.   PubMed

Nomura, A., T. Q. P. Uyeda, N. Yumoto, and Y. Tatsu (2006).
Photo-control of kinesin-microtubule motility using caged peptides derived from the kinesin C-terminus domain,
ChemComm. 34:3588-3590.   PubMed

Hirabayashi, M, S. Taira, S. Kobayashi, K. Konishi, K. Katoh, Y. Hiratsuka, M. Kodaka, T. Q. P. Uyeda, N. Yumoto, and T. Kubo (2006).
Malachite green-conjugated microtubules as mobile bioprobes selective for malachite green aptamers with capturing/releasing ability.
Biotech. Bioeng. 94:473-480.   PubMed

Taira, S., Y.-Z. Du, Y. Hiratsuka, K. Konishi, M. Eguchi, T. Kubo, T. Q. P. Uyeda, N. Yumoto, and M. Kodaka (2006).
Selective detection and transport of fully matched DNA by microtubules and kinesin motor proteins.
Biotech. Bioeng. 95:533-538.   PubMed - NCBI

Hiratsuka, Y, T. Kamei, N. Yumoto, and T. Q. P. Uyeda (2006).
Three Approaches to Assembling Nano-Bio-Machines using Molecular Motors.
NanoBiotechnology, 2:101-115.


Yumura, S., M. Yoshida, V. Betapudi, L. S. Licate, Y. Iwadate, A. Nagasaki, T. Q. P. Uyeda, and T. T. Egelhoff (2005).
Multiple myosin II heavy chain kinases: Roles in filament assembly control and proper cytokinesis in Dictyostelium.
Mol. Biol. Cell 16:4256-4266.

Kanada, M., A. Nagasaki, and T. Q. P. Uyeda (2005).
Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells.
Mol. Biol. Cell 16:3865-3872.

Rivero, F., T. Muramoto, A. Meyer, H. Urushihara, T. Q. P. Uyeda, and C. Kitayama (2005).
A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa.
BMC Genomics 6:28.   free access

Siddique, Md. S. P., G. Mogami, T. Miyazaki, E. Katayama, T. Q. P. Uyeda, and M. Suzuki (2005).
Cooperative structural change of actin filaments interacting with activated myosin motor domain, detected with copolymers of pyrene-labeled actin and acto-S1 chimera protein.
Biochem. Biophys. Res. Commun. 337:1185-1191.

Siddique, Md. S. P., T. Miyazaki, E. Katayama, T. Q. P. Uyeda, and M. Suzuki (2005).
Evidence against essential roles for subdomain 1 of actin in actomyosin sliding movements.
Biochem. Biophys. Res. Commun. 332:474-481.

Du, Y.-Z., Y. Hiratsuka, S. Taira, M. Eguchi, T. Q. P. Uyeda, N. Yumoto, and M. Kodaka (2005).
Motor protein nano-biomachine powered by self-supplying ATP.
ChemComm 16:2080-2082.

Hiratsuka, Y., and T. Q. P. Uyeda (2005).
Living microtransporter by uni-directional gliding of Mycoplasma along microtracks.
Biochem. Biophys. Res. Commun. 331:318-324.

平塚祐一、上田太郎 (2005)


Asano, Y., T. Mizuno, T. Kon, A. Nagasaki, K. Sutoh, and T. Q. P. Uyeda (2004).
Keratocyte-like locomotion in amiB-null Dictyostelium cells. Cell Motil.
Cytoskeleton 59:17-27.   PubMed

Hibi, M., A. Nagasaki, M. Takahashi, A. Yamagishi, and T. Q. P. Uyeda (2004).
Dictyostelium discoideum talin A is crucial for myosin II-independent and adhesion-dependent cytokinesis.
J. Muscle Res. Cell Motil. 25:127-140.   PubMed

Nagasaki, A., and T. Q. P. Uyeda (2004).
DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium.
Mol. Biol. Cell 15:435-446.   free access

Tokumitsu, H., N. Hatano, H. Inuzuka, Y. Ishikawa, T. Q. P. Uyeda, Janet L. Smith, and R. Kobayashi (2004).
Regulatory Mechanism of Dictyostelium Myosin Light Chain Kinase A.
J. Biol. Chem. 279:42-50.   free access

Uyeda, T. Q. P., A. Nagasaki, and S. Yumura (2004).
Multiple Parallelisms in Animal Cytokinesis.
Internatl. Rev. Cytol. 240: 377-432.   PubMed

Uyeda, T. Q. P., and A. Nagasaki. (2004).
Variations on a Theme - the Many Modes of Cytokinesis.
Curr. Opin. Cell Biol. 16:55-60.   PubMed


Kitayama, C., and T. Q. P. Uyeda (2003).
ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum.
J. Cell Sci. 116:711-723.   free access

Ito, K., T. Q. P. Uyeda, Y. Suzuki, K. Sutoh, and K. Yamamoto (2003).
Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin.
J. Biol. Chem. 278:31049-31057.   free access

Yumura, S., and T. Q. P. Uyeda (2003).
Myosins and cell dynamics in cellular slime molds.
Internatl. Rev. Cytol. 224:173-225.   PubMed




Shu, S., C. A. Parent, T. Q. P. Uyeda, and E. D. Korn (2002).
Tail chimeras of Dictyostelium myosin II support cytokinesis and other myosin II activities but not full development.
J. Cell Sci. 115:4237-4249.   free access

Nagasaki, A., G. Itoh, S. Yumura, and T. Q. P. Uyeda (2002).
A novel myosin II heavy chain kinase (MHCK C) involved in disassembly of myosin II filaments in contractile rings and efficient cleavage in mitotic Dictyostelium cells.
Mol. Biol. Cell 13:4333-4342.   free access

Nagasaki, A., E. De Hostos, and T. Q. P. Uyeda (2002) Genetic and morphological evidence for two parallel pathways of cell cycle-coupled cytokinesis in Dictyostelium.
J. Cell Sci. 115: 2241-2251.   free access

Yokoyama, K., Y. Hiratsuka, E. Akimaru, K. Hirose, T. Q. P. Uyeda, and M. Suzuki (2002).
Design and functional analysis of acto-myosin motor domain chimera proteins.
Biochem. Biophys. Res. Commun. 299:825-831.   PubMed

Uyeda, T. Q. P., B. Patterson, L. Mendoza, and Y. Hiratsuka (2002).
Amino acids 519-524 of Dictyostelium myosin II form a surface loop that aids actin binding by facilitating a conformational change.
J. Muscle Res. Cell Motil. 23:685-795.   PubMed

Uyeda, T. Q. P., K. Tokuraku, K. Kaseda, M. R. Webb, and B. Patterson (2002).
Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.
Biochemistry 30: 9525-9534.   PubMed

Onuki, R., A. Nagasaki, H. Kawasaki, T. Baba, T. Q. P. Uyeda, and K. Taira (2002).
Confirmation by FRET in individual cells of absence of significant amyloid β-mediated caspase 8 activation.
Proc. Natl. Acad. Sci. USA. 99:14716-14721.   free access

Nagasaki, A., M. Hibi, Y. Asano, and T. Q. P. Uyeda (2002).
Genetic approaches to dissect the mechanism of two distinct pathways of cell cycle-coupled cytokinesis in Dictyostelium.
Cell Struct. Funct. 26:585-591.   free access


Takahashi, M., K. Takahashi, Y. Hiratsuka, K. Uchida, A. Yamagishi, T. Q. P. Uyeda, and M. Yazawa (2001).
Functional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II.
J. Biol. Chem. 276:1034-1040.   free access

Tokuraku, K., and T. Q. P. Uyeda (2001).
Phalloidin affects the myosin-dependent sliding velocities of actin filaments in a bound-divalent cation dependent manner.
J. Muscle Res. Cell Motil. 22:371-378.   PubMed

Hiratsuka, Y., T. Tada, K. Oiwa, T. Kanayama, and T. Q. P. Uyeda (2001).
Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks.
Biophys. J. 81:1555-1561.   free access


Fukui,Y., T. Q. P. Uyeda, C. Kitayama, and S. Inoue (2000).
How well can an amoeba climb?
Proc. Natl. Acad. Sci. USA. 97:10020-10025.   free access

Liu, X., K. Ito, R. J. Lee, and T. Q. P. Uyeda (2000).
Involvement of tail domains in regulation of Dictyostelium myosin II.
Biochem. Biophys. Res. Commun. 271:75-81.   PubMed

「モデル生物:細胞性粘菌」(前田靖男編)pp.74-98. アイピーシー、東京。

Uyeda, T. Q. P., C. Kitayama, and S. Yumura (2000).
Myosin II-independent cytokinesis in Dictyostelium: its implications and mechanism.
Cell Struct. Funct. 25:1-10.   free access

Uyeda, T. Q. P., and S. Yumura (2000).
Molecular biological approaches to study myosin functions in cytokinesis of Dictyostelium.
Microscopy Res. Tech., 49:136-144.   PubMed


Fukui,Y., T. Q. P. Uyeda, C. Kitayama, and S. Inoue (1999).
Migration forces in Dictyostelium measured by centrifugal DIC microscopy.
Biol. Bull. 197:260-262.

Shu, S., R. J. Lee., J. M. LeBlanc-Straceski, and T. Q. P. Uyeda (1999).
Role of myosin II tail sequences in its function and localization to the cleavage furrow.
J. Cell Sci. 112: 2195-2201.   free access

Knetsch, M. L. W., T. Q. P. Uyeda, and D. J. Manstein (1999).
Disturbed communication between actin- and nucleotide-binding sites in a myosin II with truncated 50/20-kDa junction.
J. Biol. Chem. 274: 20133-20138.   free access

Ito, K., X. Liu, E. Katayama, and T. Q. P. Uyeda (1999).
Cooperativity between two heads of Dictyostelium myosin II in in vitro motility and ATP hydrolysis.
Biophys. J. 76: 985-992.   free access

Patterson B., 上田太郎(1999).
細胞工学18: 1631-1640。


Liu, X., K. Ito, S. Morimoto, A. Hikkoshi-Iwane, T. Yanagida and T. Q. P. Uyeda (1998).
Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation.
Proc. Natl. Acad. Sci. USA. 95: 14124-14129.   free access


Yumura, S., and T. Q. P. Uyeda (1997).
Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells.
Mol. Biol. Cell 8: 2089-2099.

Yumura, S., and T. Q. P. Uyeda (1997).
Myosin II can be localized to the cleavage furrow and to the posterior region of Dictyostelium amoebae without control by phosphorylation of myosin heavy and light chains.
Cell Motil. Cytoskeleton 36:313-322.

組換えミオシンモーター - 機能改変から新機能構築へ.

Uyeda, T. Q. P., and M. A. Titus (1997).
The myosins of Dictyostelium. In Dictyostelium - A model system for cell and developmental biology.
Universal Academy Press, Tokyo.


Uyeda, T. Q. P., P. D. Abramson, and J. A. Spudich (1996).
The neck region of the myosin motor domain acts as a lever arm to generate movement.
Proc. Natl. Acad. Sci. USA. 93:4459-4464.

上田太郎,R. B. Patterson (1996).

Uyeda, T. Q. P. (1996).
Ultra-fast Chara myosin: A test case for the swinging lever arm model for force production by myosin.
J. Plant Res. 109:231-239.


Spudich, J. A., J. Finer, B. Simmons, K. Ruppel, B. Patterson, T. Uyeda (1995).
Myosin structure and function.
Cold Spring Harbor Symp. Quant. Biol. 60:783-791.


Uyeda, T. Q. P. (1994).
Three recent breakthroughs in molecular motor research: recombinant myosin, monomolecular in vitro motility assay and atomic structure of S1.
Materials Sci. Eng. C2:1-11.

Uyeda, T. Q. P., K. M. Ruppel, and J. A. Spudich (1994).
Enzymatic activities correlate with chimeric substitutions at the actin-binding face of myosin.
Nature 368:567-569.


Uyeda, Taro Q. P., and J. A. Spudich (1993).
A functional myosin II lacking a regulatory light chain binding site.
Science, 262:1867-1870.


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